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Related Concept Videos

Septins01:19

Septins

Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
Role of Septins01:02

Role of Septins

Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
Colonisation of Pathogens01:25

Colonisation of Pathogens

Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...

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Related Experiment Video

Updated: Jun 26, 2026

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
11:50

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Published on: June 23, 2022

Septins regulate bacterial entry into host cells.

Serge Mostowy1, To Nam Tham, Anne Danckaert

  • 1Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris, France.

Plos One
|January 16, 2009
PubMed
Summary
This summary is machine-generated.

Septins, including SEPT2, SEPT9, and SEPT11, are crucial for invasive bacterial entry into mammalian cells. SEPT2 plays a key role in bacterial invasion and signaling pathways.

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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

Invasion of Human Cells by a Bacterial Pathogen
07:15

Invasion of Human Cells by a Bacterial Pathogen

Published on: March 21, 2011

Related Experiment Videos

Last Updated: Jun 26, 2026

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
11:50

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Published on: June 23, 2022

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

Invasion of Human Cells by a Bacterial Pathogen
07:15

Invasion of Human Cells by a Bacterial Pathogen

Published on: March 21, 2011

Area of Science:

  • Cell Biology
  • Microbiology
  • Biochemistry

Background:

  • Septins are conserved GTPases forming filaments essential for cellular processes like cytokinesis.
  • SEPT9 was previously identified associated with phagosomes containing InlB-coated beads.

Purpose of the Study:

  • To investigate the function of septins during the entry of invasive bacteria into non-phagocytic mammalian cells.
  • To elucidate the specific roles of SEPT2, SEPT9, and SEPT11 in bacterial invasion.

Main Methods:

  • Investigated septin recruitment at the site of bacterial entry using techniques like siRNA depletion.
  • Utilized Förster Resonance Energy Transfer (FRET) to analyze Met-dependent signaling.
  • Employed InlB-coated beads to study bacterial entry mechanisms.

Main Results:

  • SEPT9, SEPT2, and SEPT11 were recruited as collars next to actin at the entry sites of Listeria and Shigella.
  • SEPT2 depletion significantly decreased bacterial invasion, confirming septins' role in particle entry.
  • SEPT2 depletion impaired InlB-mediated Met-dependent signaling.

Conclusions:

  • Septins, particularly SEPT2, play novel and essential roles in bacterial entry into mammalian cells.
  • These findings distinguish the specific functions of septins and actin in the process of bacterial invasion.
  • SEPT2 is implicated in regulating host cell signaling pathways during bacterial infection.