Related Experiment Video
Updated: Jun 26, 2026
Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine
Published on: October 7, 2014
The detection of possible sensor histidine kinases regulating citrate/malate metabolism from the bovine rumen
W Sun1, M Mitsumori, A Takenaka
1College of Animal Science, Northwest Agriculture and Forestry University, Yangling, Shaanxi, China.
Aims:
To detect sensor histidine protein kinases (HPKs) similar to accessory gene regulator C (AgrC) from the rumen microbial ecosystem.
Methods And The Results:
Genes related to sensor HPKs were amplified by PCR using two pairs of agrC-specfic primers from DNA extracted from bovine rumen contents. The PCR products were cloned, sequenced and phylogenetically analysed. It appeared that two sequences were HPKs.
Conclusions:
Although amino acid sequences deduced from the nucleotide sequences obtained in this study showed high similarities with sensor HPKs responding to citrate or C(4)-dicarboxylates, they did not show high similarities with AgrC.
Significance And Impact Of The Study:
This study revealed the presence in the rumen of sensor HPKs responding to citrate or C(4)-dicarboxylates, which could stimulate rumen fermentation. Therefore, it has been shown that citrate or C(4)-dicarboxylate metabolism is partially regulated by a two-component regulatory system in some rumen bacteria.
More Related Videos
08:29Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
Published on: November 30, 2018
09:31Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach
Published on: October 7, 2025
