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Gene expression profiling of alveolar soft-part sarcoma (ASPS)
Luke H Stockwin1, David T Vistica, Susan Kenney
1Screening Technologies Branch, Developmental Therapeutics Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA. Stockwin@ncifcrf.gov
BMC Cancer
|January 17, 2009
Summary
Alveolar soft-part sarcoma (ASPS) gene expression profiling reveals key molecular targets. This study identifies novel pathways in angiogenesis, metastasis, and myogenic differentiation for ASPS.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Alveolar soft-part sarcoma (ASPS) is a rare, highly vascular soft tissue sarcoma.
- It predominantly affects adolescents and young adults.
- The pathobiology of ASPS remains poorly understood.
Purpose of the Study:
- To perform the first genome-wide gene expression profiling of ASPS.
- To identify transcripts with potential diagnostic or therapeutic value.
- To gain insights into the pathobiology and cellular origin of ASPS.
Main Methods:
- RNA samples from seven ASPS patients were analyzed using high-density human microarrays.
- Gene expression data was compared to universal RNA.
- Gene ontology analysis identified key transcripts, validated by quantitative RT-PCR and immunohistochemistry.
Main Results:
- Elevated expression of transcripts related to angiogenesis, cell proliferation, metastasis, and steroid biosynthesis were identified.
- Muscle-restricted transcripts suggest a muscle cell progenitor origin.
- Validation confirmed protein expression for several key genes, including MDK, c-MET, and VEGF.
Conclusions:
- This study provides the first comprehensive gene expression profile of ASPS.
- Identified targets are involved in angiogenesis, metastasis, and myogenic differentiation.
- These findings are crucial for defining ASPS cellular origin, pathogenesis, and treatment strategies.
