[A study of potential risk of HBsAg negative with RPHA]

An experimental study of potential risk of hepatitis B virus infection was carried out on 1,074 donors who were HBsAg negative by RPHA. Those so-called "qualified donors" were reexamined by ELISA and SPRIA for HBsAg, HBeAg, anti-HBc and anti-HBc-IgM, and 89 of them examined for HBV-DNA by spot hybridization. The results showed that their positive rates of HBsAg, anti-HBc-IgM, HBeAg and HBV-DNA were 5.96%, 3.35%, 2.14% and 65.17% respectively, with a total positive rate of 9.68%. The present so-called "qualified donors" are undoubtedly playing the role of source of infection to those who need blood transfusion.