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[Content comparison of diterpenoid pigments in different processed products of Gardenia jasminoides]
Cun Zhang1, Yong-Qing Xiao, Li Li
1Institute of Chinese Materia Medica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
Objective:
To compare the contents of diterpenoid pigments in the different processed products of Gardenia jasminoides.
Method:
The separation of Crocin 1, Crocin 2, Crocin 3, Crocetin were determined simultaneously by HPLC on a kromasil C18 column at 35 degrees C with the m methanol-acetonitrile-0.3% formic acid anhydrous in gradient elution as the mobile phrase. The detection wavelength was set at 440 nm and the flow rate was 1.0 mL x min(-1).
Result:
The obtained linearity of the four components was better over 0.9995 and the average recoveries were 97.77%, 100.05% , 98.40%, 101.02%, respectively.
Conclusion:
The method is simple, accurate with good reproducibility. The results showed that the remarkable variation regulations appear among the different processed products.
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