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Optimisation of reactive dye degradation by laccase using Box-Behnken design
R O Cristóvão1, A P M Tavares, J M Loureiro
1Laboratory of Separation and Reaction Engineering (LSRE), Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, Porto, Portugal.
Environmental Technology
|January 20, 2009
Summary
Commercial laccase effectively removes reactive red 180 dye. Optimal conditions for dye decolorization (>92%) involve specific enzyme concentration and pH, with temperature being non-influential.
Area of Science:
- Biotechnology
- Environmental Science
- Enzymology
Background:
- Reactive textile dyes pose environmental challenges.
- Enzymatic decolorization offers a sustainable alternative.
- Laccase is a promising enzyme for dye degradation.
Purpose of the Study:
- To optimize the decolorization of C.I. reactive red 180 using commercial laccase.
- To develop a mathematical model for predicting decolorization efficiency.
- To identify key factors influencing dye removal.
Main Methods:
- Box-Behnken factorial design with three factors.
- Response Surface Methodology (RSM) for optimization.
- Mathematical modeling to assess factor interactions.
Main Results:
- Enzyme concentration and pH were principal factors affecting decolorization.
- Dye degradation was independent of temperature.
- Optimized conditions predicted >92% decolorization.
- Experimental validation achieved 93% +/- 1.5% dye removal.
Conclusions:
- Laccase is effective for decolorizing reactive red 180.
- RSM successfully optimized the enzymatic decolorization process.
- The developed model accurately predicts decolorization efficiency.
