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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Related Experiment Video

Updated: Jun 26, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Selective gene amplification for high-throughput sequencing.

Ting Ni1, Han Wu, Shen Song

  • 1Institute for Genome Sciences & Policy, Department of Cell Biology, Duke University Medical Center, 101 Science Drive, Durham, NC 27708, USA.

Recent Patents on DNA & Gene Sequences
|January 20, 2009
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing offers cost-effective genomic analysis. This review covers patents for selective gene amplification, enabling deep sequencing of targeted genomic regions for biomedical research.

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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

Area of Science:

  • Genomics
  • Biomedical Research
  • Molecular Biology

Background:

  • Next-generation sequencing (NGS) technologies have significantly reduced the cost of genomic sequencing.
  • The complexity of the human genome prevents routine whole-genome re-sequencing.
  • Targeted deep sequencing requires methods to enrich specific genomic regions.

Purpose of the Study:

  • To review patents related to selective gene amplification techniques.
  • To highlight methods for enriching target genomic regions for NGS.
  • To underscore the utility of these techniques in biomedical research.

Main Methods:

  • Review of patent literature concerning genomic enrichment strategies.
  • Focus on methods applicable to Next-Generation Sequencing workflows.
  • Analysis of techniques for isolating and amplifying specific DNA sequences.

Main Results:

  • Identification of various patented methods for targeted genomic enrichment.
  • Demonstration of the feasibility of deep sequencing specific regions using these methods.
  • Highlighting the role of these patents in advancing biomedical research.

Conclusions:

  • Patented gene amplification techniques are crucial for enriching target regions for NGS.
  • These methods facilitate cost-effective deep sequencing of specific genomic areas.
  • The reviewed technologies support advancements in basic and translational biomedical research.