Effects of lead on kinetics of 3H-dopamine uptake by rat brain synaptosomes
M J Boykin1, C S Chetty, B Rajanna
1Division of Natural Sciences, Selma University, Alabama 36701.
Abstract:
The effects of lead on 3H-dopamine (3H-DA) uptake in rat brain synaptosomes were studied. Pb inhibited 3H-DA uptake in vitro and in vivo in a concentration-dependent manner. Altered pH versus 3H-DA uptake demonstrated comparable inhibition in buffered acidic, neutral, and alkaline pH ranges, and higher inhibition was observed in neutral pH. Kinetic studies of ATP activation of 3H-DA uptake indicated competitive inhibition by Pb. The inhibition of 3H-DA uptake followed the Na+ concentrations. The results indicate that the Pb inhibition of 3H-DA uptake is pH, ATP, and Na+ dependent.
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