Interference of paracetamol (acetaminophen) with a commercially available high-performance liquid chromatography analysis of serotonin leading to falsely low serotonin levels
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Updated: Jun 26, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Albrecht Pfäfflin1, Karsten Müssig, Erwin Schleicher
1Department of Internal Medicine IV, Clinical Chemistry Unit, University Hospital of Tübingen, Tübingen, Germany. albrecht.pfaefflin@med.uni-tuebingen.de
Abstract:
Serotonin is frequently analysed by high-performance liquid chromatography (HPLC) with electrochemical detection. However, the accuracy of these methods may be affected by the presence of certain drugs. We describe for the first time the interference of paracetamol in therapeutic dosages in a routine HPLC method for serotonin determination in vivo and in vitro. The retention time coincides with N-methylserotonin used as an internal standard in this method. Erroneous increases of the internal standard will lead, if not recognized and corrected, to falsely low serotonin determinations.
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