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Published on: July 21, 2014
Two-component signal transduction systems in Streptomyces and related organisms studied using DNA comparative
1Department of Life Science, National Yang-Ming University, Beitou, Taipei, Taiwan.
Antonie Van Leeuwenhoek
|January 20, 2009
Summary
Two component systems (TCSs) are abundant in Streptomyces, likely an evolutionary adaptation to soil environments. Analysis reveals core, species-specific, and general Actinomycetales TCSs, with differential evolution rates suggesting varied environmental responses.
Area of Science:
- Microbiology
- Genomics
- Evolutionary Biology
Background:
- Two component sensor-response regulator systems (TCSs) are prevalent in Streptomyces and other Actinomycetales, more so than in most eubacteria.
- Their abundance is hypothesized to be an evolutionary adaptation to the variable soil environments inhabited by Streptomyces.
Purpose of the Study:
- To identify a core group of TCSs within Streptomyces species.
- To differentiate TCSs into those common to Actinomycetales, specific to Streptomyces, and unique to individual species.
- To investigate the evolutionary dynamics of TCSs, including the role of horizontal transfer and the co-evolution of cognate pairs.
Main Methods:
- DNA/DNA genome microarray analysis was employed to compare 14 Streptomyces species and a related genus against Streptomyces coelicolor.
- Comparative genomics was used to analyze syntenous and non-syntenous TCSs across different Actinomycetales genomes.
- Phylogenetic analysis was implicitly used to infer evolutionary relationships and patterns.
Main Results:
- A core group of TCSs common to Streptomyces was identified.
- TCSs were categorized into Actinomycetales-general, Streptomyces-specific, and species-specific groups.
- Horizontal gene transfer was found to play a minor role in the evolution of the analyzed TCS complement.
- Cognate TCS pairs exhibit differential evolutionary rates, suggesting varied responses to environmental changes.
Conclusions:
- The study provides a detailed classification of TCSs in Streptomyces and related bacteria.
- Differential evolution rates of TCS components offer insights into adaptive evolutionary strategies.
- The findings identify potential targets for further functional studies using various genetic manipulation techniques.
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