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Related Experiment Video

Updated: Jun 26, 2026

Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors

Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors

Published on: November 15, 2016

A simple portable electroluminescence illumination-based CCD detector.

Yordan Kostov¹, Nikolay Sergeev, Sean Wilson

¹Center for Advanced Sensor Technology, University of Maryland Baltimore County, Baltimore, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 20, 2009

Summary

This summary is machine-generated.

We developed a simple, inexpensive Electroluminescence-charged-coupled device (EL-CCD) detector for fluorescence and colorimetric assays. This portable system achieves sensitive detection levels comparable to commercial instruments.

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Area of Science:

Biochemistry
Analytical Chemistry
Biotechnology

Background:

Fluorescence and colorimetric assays are crucial for detecting various analytes.
Existing detection systems can be expensive or lack portability.
There is a need for cost-effective, field-deployable detection solutions.

Purpose of the Study:

To describe a novel, portable, and inexpensive Electroluminescence-charged-coupled device (EL-CCD) based detector.
To evaluate the performance of the EL-CCD detector for fluorescence and colorimetric assays.
To demonstrate the system's adaptability for diverse assay formats.

Main Methods:

The detector utilizes an Electroluminescence (EL) panel for excitation and a cooled CCD camera for emission monitoring.
A portable, battery-operated system controlled by a laptop computer was designed.
The system was tested using a FITC-labeled peptide substrate for botulinum neurotoxin serotype A (BoNT-A) detection.

Main Results:

The EL-CCD detector achieved a detection level of 1.25 nM for the fluorogenic peptide substrate.
Performance was comparable to a commercial photomultiplier-based plate fluorometer.
The multichannel system enabled simultaneous testing of nine samples within 1 minute.

Conclusions:

The developed EL-CCD detector is a simple, portable, and cost-effective solution for fluorescence and colorimetric assays.
Its modular design allows for easy adaptation to various assays, labels, and formats.
The system offers a viable alternative to existing commercial detection platforms.