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Updated: Jun 26, 2026
08:45
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
DNA fingerprinting Candida species.
1Department of Biological Sciences, The University of Iowa, Iowa City, IA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 20, 2009
Summary
Assessing genetic relatedness of fungal infections is crucial for identifying origins and understanding drug resistance. This study details two high-resolution methods, Southern blot hybridization and multilocus sequence typing (MLST), for effective strain differentiation and clade grouping.
Area of Science:
- Mycology
- Infectious Diseases
- Genetics
Background:
- Identifying the genetic relatedness of infection isolates is essential for tracing origins.
- Drug resistance in fungal strains may be linked to specific clades and anatomical sites.
- Accurate methods are needed to distinguish highly related or unrelated fungal strains and assign them to clades.
Purpose of the Study:
- To provide detailed procedures for two highly effective methods for genetic fingerprinting of infectious fungi.
- To enable researchers to differentiate fungal strains with high resolution.
- To facilitate the grouping of fungal strains into specific clades for further study.
Main Methods:
- Southern blot hybridization using complex probes on restriction fragments.
- Multilocus sequence typing (MLST) for high-resolution genetic analysis.
- Detailed protocols for both methods are presented.
Main Results:
- Both Southern blot hybridization and MLST have proven effective in distinguishing fungal strain relatedness.
- These methods offer the resolution required for clade assignment.
- The study provides practical procedures for implementing these techniques.
Conclusions:
- Southern blot hybridization and MLST are valuable tools for analyzing the genetic relatedness of fungal isolates.
- These methods support the identification of infection origins and the study of drug resistance patterns.
- The provided procedures will aid researchers in fungal strain characterization and clade analysis.
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