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Updated: Jun 26, 2026
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
Published on: February 23, 2018
An improved method for the determination of Fc function of immunoglobulins
T Georgakopoulos1, O C Tatford, C S Tan
1Research and Development Department, CSL Bioplasma, Broadmeadows, Australia. idow@hadassah.org.il
Background And Objectives:
The Fc function assay of immunoglobulin G is performed to ensure that the biological activity of the molecule is not compromised during purification and storage. The current British Pharmacopoeia (BP) method is cumbersome, time consuming and requires a continuous source of fresh red blood cells. We have modified the technique to improve the convenience, throughput and reproducibility of the assay by using frozen red blood cells, a microtitre plate format and electronic derivative analysis for processing results.
Materials And Method:
Immunoglobulin G samples were assayed using either the BP Fc function procedure or a new procedure incorporating frozen cells, microtitre format and electronic derivative analysis.
Results:
The Fc function results demonstrated that there was no significant difference between the BP and the new improved procedure.
Conclusion:
In this study, we demonstrated that a new Fc function assay incorporating frozen red blood cells, microtitre format and derivative analysis of haemolysis curves has been shown to be comparable to the standard BP procedure. It offers a more convenient and quicker means of performing analysis of Fc function of immunoglobulins.
