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Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

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Related Experiment Video

Updated: Jun 26, 2026

Nuclear Transfer into Mouse Oocytes

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

Nuclear transfer and oocyte cryopreservation.

Ching-Chien Chang¹, Li-Ying Sung, Tomokazu Amano

¹Reproductive Biology Associates, 1150 Lake Hearn Dr, Suite 400, Atlanta, GA 30342, USA.

Reproduction, Fertility, and Development

|January 21, 2009

Summary

This summary is machine-generated.

Human therapeutic cloning faces a shortage of fresh oocytes. This review explores oocyte cryopreservation as a solution, potentially enabling regenerative medicine advancements.

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Last Updated: Jun 26, 2026

Nuclear Transfer into Mouse Oocytes

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

Area of Science:

Reproductive biology
Stem cell science
Regenerative medicine

Background:

Somatic cell reprogramming via nuclear transfer can create totipotent cells.
Therapeutic cloning offers a new avenue for regenerative medicine by deriving patient-specific embryonic stem cells.
A major limitation for human therapeutic cloning is the scarcity of fresh human oocytes.

Purpose of the Study:

To review prospective sources of human oocytes for therapeutic cloning.
To explore the potential of oocyte cryopreservation, including oocyte banking and immature oocytes.
To address challenges related to nuclear transfer using cryopreserved oocytes.

Main Methods:

Review of existing literature on oocyte cryopreservation and nuclear transfer.
Discussion of potential strategies for establishing oocyte banks.
Analysis of issues concerning the use of cryopreserved oocytes in nuclear transfer.

Main Results:

Oocyte cryopreservation presents a viable strategy to overcome the limited supply of fresh human oocytes.
Establishing oocyte banks and utilizing immature oocytes are proposed as prospective sources.
Potential issues with nuclear transfer using cryopreserved oocytes require further investigation.

Conclusions:

Cryopreserved oocytes could significantly impact regenerative medicine if their efficacy matches fresh oocytes in human therapeutic cloning.
Oocyte cryopreservation offers a promising solution to the oocyte shortage, thereby advancing therapeutic cloning.
The successful implementation of cryopreserved oocytes would be invaluable for the future of regenerative medicine.