Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating time to treatment intensification among drug naïve type 2 diabetes patients: a comparison of initial combination therapy vs step-therapy approaches.

Current medical research and opinion·2026
Same author

The development of a patient-reported outcome measuring opioid use disorder financial toxicity: Establishing content validity and reliability.

Drug and alcohol dependence reports·2026
Same author

Safety and effectiveness of immune checkpoint inhibitors in older adults with metastatic non-small cell lung cancer and pre-existing autoimmune disease - A SEER-Medicare study.

Journal of geriatric oncology·2026
Same author

Effect of initial combination therapy vs. step-therapy on adherence and persistence in drug naïve type 2 diabetes patients.

Journal of diabetes and its complications·2025
Same author

Understanding the financial barriers to treatment among individuals with opioid use disorder: a focus group study.

Harm reduction journal·2024
Same author

Real-world treatment patterns in drug naïve type 2 diabetes population: Initial combination therapy vs. sequential step-therapy.

Journal of the American Pharmacists Association : JAPhA·2024

Related Experiment Video

Updated: Jun 26, 2026

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Preface. Methods in Enzymology (fluorescence 2008)

Ludwig Brand, Michael L Johnson

    Methods in Enzymology
    |January 21, 2009
    PubMed
    Summary

    No abstract available in PubMed .

    More Related Videos

    Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
    10:57

    Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

    Published on: November 2, 2009

    Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
    07:09

    Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

    Published on: March 16, 2022

    Related Experiment Videos

    Last Updated: Jun 26, 2026

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
    09:30

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

    Published on: January 18, 2017

    Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
    10:57

    Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

    Published on: November 2, 2009

    Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
    07:09

    Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

    Published on: March 16, 2022