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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
Instrumentation for fluorescence-based fiber optic biosensors.
Richard B Thompson1, Hui-Hui Zeng, Daniel Ohnemus
1Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Methods in Enzymology
|January 21, 2009
Summary
This chapter details fluorescence-based fiber optic sensors for chemical analysis. It covers sensor construction, operation, and calibration, including metal ion determination using fluorescence changes.
Area of Science:
- Analytical Chemistry
- Optical Sensing Technologies
Background:
- Fiber optic sensors offer remote chemical detection capabilities.
- Fluorescence-based transduction provides sensitive analyte recognition.
Purpose of the Study:
- To summarize the principles, operation, and calibration of single-fiber fluorescence-based fiber optic sensors.
- To provide examples of metal ion determination in aqueous solutions.
Main Methods:
- Discusses sensor construction and operational principles.
- Details calibration procedures and instrument alignment.
- Covers noise source identification and probe preparation.
Main Results:
- Demonstrates remote measurement of chemical analytes via fluorescence changes (wavelength or lifetime).
- Provides examples of metal ion determination using fluorescence ratio and lifetime techniques.
Conclusions:
- Fluorescence-based fiber optic sensors are versatile tools for remote chemical sensing.
- Practical considerations for field and laboratory use are addressed, including long fiber applications.
