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Updated: Jun 26, 2026

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
Published on: January 26, 2024
Dissecting the splicing mechanism of the Drosophila editing enzyme; dADAR
Roberto Marcucci1, Maurizio Romano, Fabian Feiguin
1International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012, Italy.
Abstract:
In Drosophila melanogaster, the expression of adenosine deaminase acting on RNA is regulated by transcription and alternative splicing so that at least four different isoforms are generated that have a tissue-specific splicing pattern. Even though dAdar has been extensively studied, the complete adult expression pattern has yet to be elucidated. In the present study, we investigate mature transcripts of dAdar arising from different promoters. Two predominant isoforms of dAdar are expressed in gonads and dAdar is transcribed from both the embryonic and the adult promoters. Furthermore, full-length transcripts containing the alternatively spliced exon-1 are expressed in a tissue-specific manner. The splicing factor B52/SRp55 binds within the alternative spliced exon 3a and plays a role in this alternative splicing event.
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