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Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
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Gene expression differences in infected and noninfected middle ear complementary DNA libraries.

Joseph E Kerschner1, Edward Horsey, Azad Ahmed

  • 1Department of Otolaryngology and Communications Disorders, Children's Hospital of Wisconsin, 9000 W Wisconsin Ave, Milwaukee, WI 53226, USA. kersch@mcw.edu

Archives of Otolaryngology--Head & Neck Surgery
|January 21, 2009
PubMed
Summary

This study reveals genetic changes in middle ear mucosa during nontypeable Haemophilus influenzae (NTHi) infection. Key genes involved in immune and stress responses were upregulated, offering insights into otitis media pathogenesis.

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Area of Science:

  • Microbiology
  • Genetics
  • Immunology

Background:

  • Nontypeable Haemophilus influenzae (NTHi) is a common cause of otitis media (OM).
  • Genetic changes in middle ear mucosa (MEM) during OM pathogenesis are not fully understood.
  • Previous comprehensive assessments of genetic differences in NTHi-infected MEM are lacking.

Purpose of the Study:

  • To investigate genetic differences in MEM during NTHi infection.
  • To identify specific genes and biological processes affected by NTHi infection in the middle ear.
  • To establish a framework for understanding molecular changes in OM.

Main Methods:

  • Construction of complementary DNA (cDNA) libraries from NTHi-infected and noninfected chinchilla MEM.
  • Sequencing of random cDNA clones and submission to NCBI Expressed Sequence Tags database.
  • Analysis of gene expression differences using BLAST and FatiGO to identify significant biological processes.

Main Results:

  • Significant differences in gene expression were identified between infected and noninfected MEM.
  • Biological processes related to immune, stress, and wound responses were more prevalent in NTHi-infected MEM.
  • Upregulation of genes such as S100A9, SLPI, B2M, FTH1, and S100A8 was observed in NTHi-infected MEM.

Conclusions:

  • Gene expression changes, including SLPI, occur during OM pathogenesis and impact mucin production.
  • Identified genes like SLPI play a role in regulating OM.
  • The study provides a foundation for future research into middle ear molecular changes and therapeutic strategies for OM.