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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 26, 2026

The MPLEx Protocol for Multi-omic Analyses of Soil Samples
10:12

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

Published on: May 30, 2018

Sequential detergent extraction prior to mass spectrometry analysis.

Fiona M McCarthy1, Amanda M Cooksey, Shane C Burgess

  • 1Department of Basic Sciences, Mississippi State University, MS 39762, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 21, 2009
PubMed
Summary
This summary is machine-generated.

Sequential detergent extraction (SDE) enhances proteome coverage for mass spectrometry. This method identifies twice as many membrane proteins, including those with multiple transmembrane domains, offering insights into cellular localization.

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Last Updated: Jun 26, 2026

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A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

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Area of Science:

  • Proteomics
  • Cellular Biology
  • Biochemistry

Background:

  • 2D-PAGE is a common method for protein separation.
  • Increasing proteome coverage, especially for membrane proteins, remains a challenge in proteomics.
  • High-throughput methods are needed to analyze complex proteomes.

Purpose of the Study:

  • To adapt sequential detergent extraction (SDE) for high-throughput proteomics.
  • To improve the comprehensive analysis of eukaryotic cell proteomes.
  • To enhance the identification of membrane proteins and their properties.

Main Methods:

  • Adaptation of sequential detergent extraction (SDE) for liquid chromatography and electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS).
  • Application of SDE to eukaryotic cell protein extraction.
  • Analysis of extracted proteomes using high-throughput mass spectrometry.

Main Results:

  • The adapted SDE method significantly increased proteome coverage.
  • Up to twice the number of membrane proteins were identified compared to other methods.
  • Two-thirds of identified membrane proteins possessed one or more transmembrane domains.
  • SDE provided valuable data on protein physicochemical properties and sub-cellular localization.

Conclusions:

  • Sequential detergent extraction is a powerful technique for comprehensive proteome analysis.
  • This method substantially improves the identification of membrane proteins, crucial for understanding cellular functions.
  • SDE offers insights into protein localization, aiding in the study of cellular architecture and processes.