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Cyclosporin A blocks surface IgM-mediated growth inhibition in an immature B lymphoma, BKS-2
V Udhayakumar1, S Muthukkumar, B Subbarao
1Department of Microbiology and Immunology, University of Kentucky, Lexington 40536-0230.
Cyclosporin A (CSA) is an immunosuppressive drug, which blocks selective activation pathways in T and B cells. Antigen receptor-mediated signaling events have been shown to be very sensitive to CSA. Signaling through the surface IgM receptor had been shown to induce growth inhibition in immature B cell lymphoma cells. In this report, we demonstrate that CSA caused significant reversal of growth inhibition induced by an anti-mu antibody in an immature B lymphoma, BKS-2. Time-course experiments indicated that CSA was completely effective when added as late as 4 h after the addition of ligand. CSA was also found to have no direct effect on anti-mu-induced Ca2+ elevation. These results suggest that the likely target for CSA lies downstream from the initial generation of second messengers, such as Ca2+.
Cyclosporin A (CSA) is an immunosuppressive drug, which blocks selective activation pathways in T and B cells. Antigen receptor-mediated signaling events have been shown to be very sensitive to CSA. Signaling through the surface IgM receptor had been shown to induce growth inhibition in immature B cell lymphoma cells. In this report, we demonstrate that CSA caused significant reversal of growth inhibition induced by an anti-mu antibody in an immature B lymphoma, BKS-2. Time-course experiments indicated that CSA was completely effective when added as late as 4 h after the addition of ligand. CSA was also found to have no direct effect on anti-mu-induced Ca2+ elevation. These results suggest that the likely target for CSA lies downstream from the initial generation of second messengers, such as Ca2+.