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Permanent marking of colored mice using dry ice.

¹Laboratory Animal Center, Yamagata University School of Medicine, Japan.

Jikken Dobutsu. Experimental Animals

|July 1, 1991

Summary

Freeze marking with dry ice offers a simple, permanent method for identifying individual colored mice. This technique creates visible white hair spots without harming the animals, improving upon traditional identification methods.

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Area of Science:

Animal Science
Veterinary Medicine
Laboratory Animal Science

Background:

Accurate individual identification is crucial for laboratory animal studies.
Conventional marking methods can be time-consuming, invasive, or prone to loss.
Need for a reliable, non-invasive, and permanent identification technique for research mice.

Purpose of the Study:

To evaluate the efficacy of freeze marking using dry ice for permanent individual identification of colored mice.
To assess the impact of freeze marking on the animals' health and the integrity of the marking.

Main Methods:

A 5-8 mm piece of dry ice was applied to the skin of colored mice for 10 seconds.
Observation of hair regrowth and identification of white spots occurred approximately 3 weeks post-treatment.
Assessment of the marking's permanence and any discernible effects on the animals.

Main Results:

Freeze marking successfully created permanent, visible white hair spots on colored mice.
The white spots were easily recognizable, facilitating individual animal identification.
No discernible adverse effects were observed at the treatment site or on the general condition of the animals.

Conclusions:

Freeze marking with dry ice is an effective and safe method for permanent individual identification of colored laboratory mice.
This technique offers a significant improvement over conventional marking methods in terms of clarity and permanence.
The method is practical for routine use in animal facilities, enhancing research data integrity.

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