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Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics
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Two-dimensional droplet-based surface plasmon resonance imaging using electrowetting-on-dielectric microfluidics.

Lidija Malic1, Teodor Veres, Maryam Tabrizian

  • 1Biomedical Engineering Department, McGill University, Montreal, QC H3A 2B4, Canada.

Lab on a Chip
|January 22, 2009
PubMed
Summary

This study introduces a novel multichannel platform combining digital electrowetting-on-dielectric (EWOD) microfluidics with surface plasmon resonance imaging (SPRi). This innovation enables parallel detection, significantly enhancing high-throughput analysis capabilities for SPRi applications.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Surface Plasmon Resonance Imaging (SPRi) is a label-free, in-situ technique for monitoring surface-based reactions.
  • Current SPRi applications are limited by flow-cell technology, which processes samples sequentially, hindering high-throughput analysis.
  • Digital microfluidics offers potential for parallel sample handling and processing.

Purpose of the Study:

  • To develop a multichannel droplet-based SPRi platform for enhanced high-throughput analysis.
  • To overcome the limitations of sequential sample processing in conventional SPRi flow cells.
  • To integrate digital electrowetting-on-dielectric (EWOD) microfluidics with SPRi for parallel detection.

Main Methods:

  • A digital electrowetting-on-dielectric (EWOD) microfluidic device was designed and fabricated.
  • The EWOD device was coupled to a Surface Plasmon Resonance Imaging (SPRi) system.
  • Droplet manipulation and positioning on the SPRi chip were controlled using EWOD actuation.
  • Simultaneous SPRi detection of multiple, distinct samples was performed in parallel.

Main Results:

  • The integrated platform successfully demonstrated multichannel, parallel SPRi detection.
  • The digital microfluidic device enabled precise control over sample droplet positioning and dispensing.
  • The system achieved in-situ, label-free monitoring of multiple reactions concurrently.
  • The proposed solution significantly increases the throughput capability compared to traditional flow-cell SPRi.

Conclusions:

  • The developed multichannel droplet-based SPRi platform effectively addresses the throughput limitations of existing SPRi technology.
  • Integration of EWOD microfluidics with SPRi enables parallel processing and analysis of multiple samples.
  • This approach offers a promising solution for high-throughput screening and diagnostics using SPRi.