Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Clinical Significance of Antibiotic Resistance

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...

Mechanism of Antibiotic Resistance in MRSA

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...

Staphylococcal Skin Infections

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Matrix extension to bovine feces and evaluation of semi-automated DNA extraction methods for the detection of <i>Campylobacter jejuni</i> in bovine and canine fecal samples using <i>gyrA</i> PCR.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2026

Same author

Age-specific early-life gut microbiome associations with eczema and food allergies during early immune development.

Frontiers in microbiomes·2026

Same author

Understanding Equity in Cancer Prehabilitation Services in Wales: A Medical Record Review.

Cancer reports (Hoboken, N.J.)·2026

Same author

Rapid detection of human and animal respiratory viruses using Microbe Finder (MiFi<sup>®</sup>).

Frontiers in microbiology·2026

Same author

Rabies Cluster Among Steers on a Dairy Farm - Minnesota, 2024.

MMWR. Morbidity and mortality weekly report·2025

Same author

Detection of leptospiral antibodies and DNA in freshwater fish.

Frontiers in veterinary science·2025

Search research articles

Related Experiment Video

Updated: Jun 26, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

CE-based detection of methicillin-resistant Staphylococcus aureus.

Hemant Naikare¹, Akhilesh Ramachandran, David Goad

¹Texas Veterinary Medical Diagnostic Laboratory, Amarillo, TX 19106, USA. hnaikare@tvmdl.tamu.edu

Electrophoresis

|January 22, 2009

Summary

This study presents a rapid, 3-hour method for detecting methicillin-resistant Staphylococcus aureus (MRSA) in blood samples. The technique uses isothermal amplification and cycling probe reaction coupled with capillary electrophoresis-laser induced fluorescence for sensitive MRSA identification.

Related Experiment Videos

Last Updated: Jun 26, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Area of Science:

Clinical microbiology
Molecular diagnostics
Analytical chemistry

Background:

Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant threat in clinical settings.
Rapid and sensitive detection methods are essential for effective MRSA infection management.

Purpose of the Study:

To develop a capillary electrophoresis (CE)-based method for the multiplexed detection of MRSA.
To identify specific DNA sequences (femA for S. aureus and mecA for methicillin resistance).

Main Methods:

Isothermal amplification of femA and mecA genes from spiked blood samples.
Cycling probe reaction (CPR) assay using chimeric probes and RNase H.
Detection of fluorophore fragments via capillary electrophoresis-laser induced fluorescence (CZE-LIF).

Main Results:

The assay achieved a limit of detection of approximately 10(4) CFU/mL.
Accurate detection of MRSA was accomplished within a 3-hour timeframe.
Multiplexed identification of S. aureus and methicillin resistance genes was demonstrated.

Conclusions:

The developed CPR-CZE-LIF method offers a rapid and sensitive approach for MRSA detection.
This technique has potential for timely clinical diagnosis and infection control.
The assay enables simultaneous identification of bacterial species and resistance markers.