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Updated: Jun 26, 2026

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
Published on: April 10, 2020
Detection of carbonylated proteins in two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis
Rukhsana Sultana1, Shelley F Newman, Quanzhen Huang
1Department of Chemistry, Center of Membrane Sciences, University of Kentucky, Lexington, KY, USA.
Abstract:
Protein carbonyls are an index of protein oxidation which, in turn, reflects the interplay of oxidative stress and degradation of oxidatively modified proteins. Protein carbonyls are increased in brain proteins in aging and age-related neurodegenerative disorders, including Alzheimer's disease. In this chapter, we outline methods to detect protein carbonyls following two dimensional-based separation of brain proteins.
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