Related Experiment Video
Updated: Jun 26, 2026

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia
Published on: April 7, 2023
[Determination of paeoniflorin in rat plasma by HPLC-MS/MS and its pharmacokinetics]
1Jiangsu Provincial Institute of Traditional Chinese Medicine, Nanjing 210028, China. wujie613@hotmail.com
Objective:
An HPLC-MS/MS assay was established to determine paeoniflorin in rat plasma and be used to investigate the effect of pharmacokinetics of paeoniflorin when co-administrated Radix Angelicae Sinensis and Radix Paeoniae Rubra.
Method:
HPLC-MS/MS was performed in the multiple reaction monitoring (MRM) mode using target ions at m/z 450 --> m/z 327 for paeoniflorin and m/z 388 --> m/z 225 for jasminoidin. A single dose of Radix Paeoniae Rubra alone and with Radix Angelicae Sinensis was given to rats by ig, the dosage of paeoniflorin was 294.78 mg x kg(-1). Concentrations of paeoniflorin in rat plasma were determined by HPLC-MS/MS assay and main pharmacokinetic parameters were estimated.
Result:
The mainly pharmacokinetic parameters of paeoniflorin when administrated Radix Paeoniae Rubra only were as follows: C(max) (1.55 +/- 0.53) mg x L(-1), T(max) (0.9 +/- 0.3) h, t 1/2 (1.51 +/- 0.63) h, MRT (3.08 +/- 0.74) h, AUC(0 --> tau) (4.68 +/- 0.85) mg x h(-1) x L(-1). When the two drugs administrated together, the corresponding parameters were (0.93 +/- 0.42) mg x L(-1), (1.5 +/- 0.8) h, (3.08 +/- 1.79) h, (5.19 +/- 1.95) h, (3.36 +/- 0.56) mg x h(-1) x L(-1), respectively. The parameters of C(max), AUC(0 --> tau) and MRT were significantly changed (P < 0.05).
Conclusion:
Radix Angelicae Sinensis can significantly affect the pharmacokinetics of paeoniflorin, the principal component of Radix Paeoniae Rubra.
More Related Videos
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
13:35A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
Related Concept Videos
Nonlinear Pharmacokinetics: Dependence of Elimination Half-Life and Dose Clearance
A study on guinea pigs examined the...
Measurement of Bioavailability: Pharmacokinetic Methods
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses
Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Metabolism
Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow