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A novel assay for the in vivo study of Schwann cells
1Louisiana State University School of Veterinary Medicine, Department of Veterinary Anatomy and Fine Structure, Baton Rouge 70803.
Experimental Neurology
|October 1, 1991
Abstract:
An in vivo assay was developed for long-term analyses of Schwann cells at the single cell level. Schwann cells were isolated from sciatic nerves and labeled with the fluorescent gold label Fluoro-Gold. Cells were then transplanted into severed sciatic nerves of young adult rats. Gold-labeled Schwann cells, identified by double-labeling with S100 antibodies, were observed up to 10 mm away from the transplant site and after 90 and 120 days of survival in vivo.