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Transgenic regulation in laboratory animals
1Institut für Molekularbiologie II der Universität Zürich, Switzerland.
Experientia
|September 15, 1991
Summary
This review explores transgene expression regulation in lab animals, detailing gene transfer methods and cis-acting elements. It discusses current advancements and future directions in surrogate genetics, alongside technical and ethical challenges.
Area of Science:
- Molecular Biology
- Genetics
- Animal Models
Background:
- Understanding transgene expression regulation is crucial for laboratory animal research.
- Gene transfer techniques have evolved significantly, impacting experimental outcomes.
- Cis-acting elements and chromatin state play vital roles in controlling gene expression.
Purpose of the Study:
- To summarize current knowledge on regulating transgene expression in laboratory animals.
- To review gene transfer methods, their advantages, and limitations.
- To discuss the role of regulatory elements and chromatin in gene expression control.
Main Methods:
- Historical overview of gene transfer techniques.
- Description of popular gene transfer methods, including targeted gene replacement.
- Analysis of cis-acting elements (promoters, enhancers, silencers, DCRs) and chromatin influence.
Main Results:
- Different gene transfer approaches exhibit varying gene expression patterns.
- Specific examples illustrate the function of cis-acting elements and chromatin states.
- Recent advancements in surrogate (reversed) genetics are presented.
Conclusions:
- The field of transgene expression regulation is dynamic with emerging techniques.
- Surrogate genetics offers new avenues for research.
- Technical and socio-ethical challenges require further attention.
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