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Transgenesis in fish.

L M Houdebine1, D Chourrout

  • 1Unité de Différenciation Cellulaire, Institut National de la Recherche Agronomique, Jouy en Josas, France.

Experientia
|September 15, 1991
PubMed
Summary

Gene transfer into fish embryos, primarily via microinjection, results in mosaic transgenic animals with stable Mendelian inheritance. Transgene expression is often poor, though some mammalian sequences show efficiency in fish cells.

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Area of Science:

  • Aquaculture and Aquatic Sciences
  • Molecular Biology
  • Genetics

Background:

  • Gene transfer into fish embryos is crucial for various research and aquaculture applications.
  • Traditional methods often face challenges due to the lack of visible pronuclei in many fish species.

Purpose of the Study:

  • To investigate the efficiency and characteristics of gene transfer into diverse fish species.
  • To analyze the integration, transmission, and expression of foreign DNA in transgenic fish.

Main Methods:

  • Microinjection of foreign DNA into early-stage fish embryos (cytoplasm or nucleus).
  • Analysis of DNA integration, copy number, and transmission patterns using Southern blot.
  • Assessment of transgene expression in various tissues of founder and offspring generations.

Main Results:

  • Transgenic animals were successfully generated across multiple fish species with varying success rates (1-50%).
  • Transgenic fish exhibited mosaicism, with integration occurring early in development and stable Mendelian transmission observed.
  • Transgene expression was generally poor, although some mammalian regulatory sequences demonstrated functional activity in fish cells.

Conclusions:

  • Gene transfer into fish embryos is feasible but results in mosaicism and variable expression levels.
  • Further research is needed to optimize gene expression and understand the factors influencing transgenesis efficiency in fish.

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