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Related Experiment Video

Updated: Jun 26, 2026

In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats
08:19

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Published on: September 27, 2017

TPRV1 expression defines functionally distinct pelvic colon afferents.

Sacha A Malin1, Julie A Christianson, Klaus Bielefeldt

  • 1Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|January 23, 2009
PubMed
Summary

Researchers identified two types of colon sensory neurons, high-frequency (HF) and low-frequency (LF) firing cells, in mice. Most LF cells express TRPV1 and are sensitized by various stimuli, suggesting a role in visceral hypersensitivity.

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Last Updated: Jun 26, 2026

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08:19

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Published on: September 27, 2017

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09:48

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Published on: March 7, 2020

Area of Science:

  • Neuroscience
  • Gastroenterology
  • Pain Research

Background:

  • Chronic visceral pain is often linked to changes in primary sensory neurons.
  • Understanding visceral pain requires comprehensive neuronal phenotypes combining function and anatomy.

Purpose of the Study:

  • To develop a novel ex vivo mouse model for studying colon sensory neurons.
  • To characterize the functional and anatomical properties of distension-responsive colon afferents.
  • To investigate the role of the capsaicin receptor, TRPV1, in colon afferent responses.

Main Methods:

  • Developed a novel ex vivo mouse physiology preparation for intracellular recordings from colon sensory neurons.
  • Performed colon distension in the presence and absence of pharmacologic agents.
  • Recovered functionally characterized afferents for histochemical analysis, including TRPV1 expression.

Main Results:

  • Identified two distinct populations of distension-responsive colon afferents: high-firing frequency (HF) and low-firing frequency (LF) cells.
  • LF cells had significantly higher distension response thresholds and were frequently sensitized by capsaicin, mustard oil, and low pH.
  • A majority of LF afferents (87%) expressed TRPV1, while only a small fraction of HF afferents (17%) did.

Conclusions:

  • Colon sensory neurons can be classified into distinct HF and LF populations based on firing frequency and response to distension.
  • TRPV1-positive LF colon afferents play a significant role in visceral hypersensitivity.
  • Targeting TRPV1-positive colon afferents may offer a therapeutic strategy for visceral pain.