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Related Concept Videos

Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
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Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
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The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

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Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
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Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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Related Experiment Video

Updated: Jun 26, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

TMAP/CKAP2 is essential for proper chromosome segregation.

Kyung Uk Hong1, Eunhee Kim, Chang-Dae Bae

  • 1Department of Molecular Cell Biology and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Jangangu, Suwon Republic of Korea.

Cell Cycle (Georgetown, Tex.)
|January 23, 2009
PubMed
Summary

Tumor-associated microtubule-associated protein (TMAP/CKAP2) is crucial for accurate chromosome segregation during cell division. Its depletion leads to genomic instability and reduced cell viability, highlighting its role beyond spindle regulation.

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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Tumor-associated microtubule-associated protein (TMAP), also known as cytoskeleton associated protein 2 (CKAP2), is upregulated in malignancies.
  • The precise cellular functions of TMAP/CKAP2, particularly in mitosis, are not fully understood.
  • Previous research suggested TMAP/CKAP2 involvement in mitotic spindle dynamics and assembly.

Purpose of the Study:

  • To investigate the role of TMAP/CKAP2 in mitosis and chromosome segregation.
  • To determine the cellular consequences of TMAP/CKAP2 depletion in mammalian cells.

Main Methods:

  • siRNA-mediated depletion of TMAP/CKAP2 in cultured mammalian cells.
  • Analysis of spindle apparatus, nuclear morphology, and nuclear lamina organization.
  • Time-lapse video microscopy to observe cellular dynamics.
  • Assessment of spindle checkpoint activity, cell viability, and chromosomal instability.

Main Results:

  • TMAP/CKAP2 knockdown did not significantly alter the mitotic spindle apparatus.
  • Depletion resulted in abnormal nuclear morphologies, nuclear lamina disorganization, and chromatin bridges.
  • Defects in chromosome segregation and reduced spindle checkpoint activity were observed.
  • Chromosome missegregation led to decreased cell viability and increased chromosomal instability.

Conclusions:

  • TMAP/CKAP2 is essential for proper chromosome segregation during mitosis.
  • TMAP/CKAP2 plays a critical role in maintaining genomic stability.
  • The function of TMAP/CKAP2 extends beyond spindle regulation to encompass nuclear integrity and accurate cell division.