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Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy
A Grziwa1, W Baumeister, B Dahlmann
1Max-Planck-Institut für Biochemie, Martinsried, Germany.
FEBS Letters
|September 23, 1991
Abstract:
The subunit topography of the Thermoplasma acidophilum proteasome was determined by immunoelectron microscopy using monospecific antibodies directed against the two constituent subunits (alpha,beta). Anti-alpha-subunit IgG was found to bind to the outer disks of the cylinder- or barrel-shaped molecule, while the binding sites of the anti-beta-subunit IgG were mapped on the two inner rings. Probably the homologues of the two subunits in the compositionally more complex but isomorphous eukaryotic proteasomes occupy equivalent positions.