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Formation and crystallization of Thermus thermophilus 70S ribosome/tRNA complexes
G Yusupova1, M Yusupov, A Spirin
1Institute of Protein Research, Academy of Sciences of the USSR, Pushchino.
FEBS Letters
|September 23, 1991
Abstract:
70S ribosomes from Thermus thermophilus are able to form ternary complexes with N-AcPhe-tRNAPhe from either Thermus thermophilus or Escherichia coli, in the presence of a short oligo(U) of six or nine uridines. A complex of N-AcPhe-tRNAPhe/(U)9/70S ribosome from Th. thermophilus was crystallized under the same conditions used for the growth of crystals from isolated ribosomes (S.D. Trakhanov, et al., (1987) FEBS Lett. 220, 319-322).