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Published on: February 1, 2018
A set of piezoelectric biosensors using cholinesterases
Carsten Teller1, Jan Halámek, Alexander Makower
1Department of Analytical Biochemistry, University of Potsdam, Potsdam, Germany.
Abstract:
Piezoelectric sensors have become a versatile tool in biosensorics to study protein-protein and protein-small molecule interactions. Here we present theoretical background on piezoelectric sensors and instructions, how to modify their surface with various recognition elements for cholinesterases. These recognition elements comprise an organophosphate (paraoxon), a cocaine derivative (BZE-DADOO), and a tricyclic, aromatic compound (propidium). Additionally, a guide to the kinetic evaluation of the obtained binding curves is given in this chapter.
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