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Updated: Jun 26, 2026

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Biosensor detection systems: engineering stable, high-affinity bioreceptors by yeast surface display.

Sarah A Richman1, David M Kranz, Jennifer D Stone

  • 1Department of Biochemistry, University of Illinois, Urbana, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 23, 2009
PubMed
Summary
This summary is machine-generated.

Yeast surface display enables directed evolution of bioreceptors for enhanced biosensor applications. This method improves binding affinity and stability, crucial for sensitive and reusable detection tools.

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Protein Engineering by Yeast Surface Display
05:49

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Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
10:54

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

Area of Science:

  • Biotechnology
  • Biomolecular Engineering
  • Biosensor Development

Background:

  • Biosensors require highly specific and stable bioreceptors for accurate ligand detection.
  • Naturally occurring bioreceptors often lack sufficient affinity or stability for practical biosensor use.
  • Improving bioreceptor performance is key to advancing sensitive and reusable detection technologies.

Purpose of the Study:

  • To detail the application of yeast surface display for directed evolution of bioreceptors.
  • To enhance both the stability and ligand-binding affinity of bioreceptor molecules.
  • To demonstrate the production of improved bioreceptors for biosensing applications.

Main Methods:

  • Utilizing yeast surface display for directed evolution of binding molecules.
  • Engineering bioreceptors, including single-chain antibodies and T cell receptors.
  • Selecting for improved stability and increased ligand affinity through evolutionary techniques.

Main Results:

  • Achieved up to a million-fold increase in ligand-binding affinity for engineered bioreceptors.
  • Successfully produced stabilized bioreceptor molecules.
  • Demonstrated the expression of stable binding molecules in E. coli.

Conclusions:

  • Yeast surface display is a powerful technique for optimizing bioreceptors for biosensors.
  • Directed evolution via yeast display significantly enhances bioreceptor affinity and stability.
  • This approach facilitates the development of advanced, high-performance biosensing tools.