Visualization of spatiotemporal differentiation of dopaminergic interneurons in adult mouse olfactory bulb using...

Olfactory bulb (OB) interneurons, predominantly periglomerular (PG) and granule (GR) cells, are derived from neural stem cells in the subventricular zone (SVZ) throughout life and migrate to the OB in the rostral migratory stream (RMS). In the adult superficial GR layer transgene expression was found, either enhanced green fluorescent protein or LacZ reporter driven by a 9 kb tyrosine hydroxylase (TH) promoter, that marked the dopamine (DA) phenotype. To demonstrate that the reporters and endogenous TH were similarly regulated expression of both parameters was shown to decline in the OB PG cells ipsilateral to odor deprivation produced by adult unilateral naris closure. The present findings suggested that DAergic differentiation might begin prior to progenitors reaching a PG position despite evidence that TH protein expression occurred only after PG cells received olfactory afferent stimulation. Of many genes previously hypothesized to regulate OB DA expression, regulated expression of the orphan receptor, Nurr1, but not the homeobox-containing genes, Dlx-1 and -2, was consistent with a role in regulation of the DA phenotype in adult mice OB. The studies show that transgenic lines are useful for analyzing spatiotemporal regulation by both intrinsic (programmed) and environmental factors in the neurogenesis of adult mouse OB DAergic interneurons.