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[Effect of Lactobacillus rhamnosus GG on immune response: experiment with autoimmune mice induced by Campylobacter
1Wuhu Center for Disease Control and Prevention, Wuhu 241000, China. zhangqiong36@sohu.com
Objective:
To investigate the immune effects of Lactobacillus rhamnosus GG (LGG) on immune response.
Methods:
Fourty ICR mice were randomly divided into 4 groups, normal control (N) group, model (M) group, Lactobacillus (LGG) group, and hydrocortisone (HC) group. The mice were induced by Campylobacter jejuni with complete Freund's adjuvant as autoimmune animal model except N group. From the fifteenth day after primary immunization, each group was given different administration through peritoneum route for 7 days. Twenty-five days after the model establishment, the mice were killed. Then the blood sample was collected the undergo ELISA to detect the level of ds-DNA antibody and interferon (IFN)-gamma. The proliferation activities of the B and T cells were examined. The livers and kidneys were taken out to undergo microscopy.
Results:
The ds-DNA antibody levels of the HC and LGG groups were not significant different from that of the M group (both P > 0.05). The proliferation activities of T and B cells and IFN-gamma level of the groups LGG were all significantly lower than those of the group M [0.42 +/- 0.05 vs 0.60 +/- 0.09, 0.43 +/- 0.05 vs 0.60 +/- 0.09, (184 +/- 16) vs (195 +/- 6) pg/ml, all P < 0.01].
Conclusion:
LGG inhibits the immune response induced by CJ to a certain degree.
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