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Updated: Jun 26, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes
Published on: May 4, 2020
A LC-MS method allowing the analysis of HMX and RDX present at the picogram level in natural aqueous samples without
Olivier Vigneau1, Xavier Machuron-Mandard
1Commissariat à l'Energie Atomique, Centre DAM Ile-de-France, Bruyères-le-Châtel, 91297 Arpajon Cedex, France.
Abstract:
The introduction of chloroform into the nebulising gas of a LC/MS electrospray interface (ESI), in a perfectly controlled way, leads to the formation of intense adducts ([M+Cl](-)) when a mobile phase containing HMX (1,3,5,7-tetranitro-1,3,5,7-tetrazacyclooctane or octogen) and RDX (1,3,5-trintro-1,3,5-triazacyclohexane or hexogen) is eluted. This LC/MS method allows the direct analysis of aqueous samples containing HMX and RDX at the pictogram level without a concentration step. The method is used to determine HMX and RDX concentrations in ground water samples from a military site.
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