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A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Diimine ligand as a novel chemiluminescence enhancer of luminol-containing compounds
Chaivat Smanmoo1, Mutsumi Yamasuji, Tomoko Sagawa
1Faculty of Pharmaceutical Sciences, School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki 852-8521, Japan.
Abstract:
A series of diimine ligands (DLs) have been synthesized and evaluated for their non-enzymatic chemiluminescence (CL) enhancement of isoluminol or luminol-containing compounds. Of the DLs, N,N'-bis(m-hydroxylbenzylidene)propylenediamine (DL 10) was found to greatly enhance their CLs approximately 40 folds for isoluminol, 10 folds for luminol and 6 folds for a luminol-containing polymer. The CL enhancement of the compounds was observed in the presence of CH(3)CN, H(2)O(2), tetra-n-propylammonium hydroxide (TPA), and Fe (III) ion. The possible mechanism of this CL enhancement was discussed on the basis of the chelate formation of the ligand and the metal ions.
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