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Related Experiment Videos

Altered expression of glucose transporter isoforms with aging in rats--selective decrease in GluT4 in the fat tissue

J L Lin1, T Asano, Y Shibasaki

  • 1Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

Diabetologia
|July 1, 1991
PubMed
Summary

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Aging impairs glucose tolerance by reducing glucose transporter proteins, particularly GLUT4, in fat and muscle tissues. This decline is linked to decreased synthesis in fat and reduced translation or stability in muscle.

Area of Science:

  • Metabolic regulation and aging research.

Background:

  • Aging is associated with glucose intolerance.
  • Glucose transporters play a crucial role in glucose uptake and metabolism.

Purpose of the Study:

  • To investigate the cellular mechanisms of age-related glucose intolerance.
  • To examine changes in glucose transporter (GLUT) protein and mRNA levels in young versus aged rats.

Main Methods:

  • Comparison of GLUT4, GLUT2, and GLUT1 protein and mRNA levels in adipose, skeletal muscle, and liver tissues.
  • Analysis of aged (20-month-old) and young (7-week-old) rats.

Main Results:

  • Aged rats showed decreased GLUT4 protein in epididymal fat and gastrocnemius muscle.
  • GLUT4 mRNA decreased in fat, suggesting reduced synthesis, while it increased in muscle, indicating decreased translational efficiency or protein stability.

Related Experiment Videos

  • Aged rats also exhibited decreased GLUT2 protein and mRNA in the liver.

    • Conclusions:

    • Aging leads to reduced glucose transporter expression and function, contributing to glucose intolerance.
    • Cellular mechanisms for GLUT4 regulation differ between adipose tissue and skeletal muscle in aged rats.