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Published on: November 11, 2022
[Study on HPLC fingerprint of Prunella vulgaris]
Wei-Guang Sun1, Xue-Hong Ke, Yuan Li
1Guangzhou Xingqun Pharmaceutical Ltd. Co., Guangzhou 510288, China.
Objective:
To establish HPLC fingerprint of Prunella vulgarise for quality control of the herbal medicine.
Method:
A sunfire C18 analytical column was used. The mobile phase A was 1% acetic acid, and mobile phase B was methanol. The elution was in gradient mode and detection wavelength was set at 290 nm. The flow rate was 1.0 mL x min(-1) and the column temperature at 30 degrees C. The analysis time was 60 min.
Result:
The similarity of 10 batches of P. vulgaris was not lower than 0.810. The fingerprints of the herbal medicine were classified P. vulgaris on the results of cluster analysis.
Conclusion:
This method is available for quality evaluation and control the quality of P. vulgaris.
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