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Updated: Jun 26, 2026

Optimizing Tubulin Yield from Porcine Brain Tissue
Published on: October 11, 2024
[Effect of nobiletin on tubulin polymerization in vitro]
Yi Liu1, Ke-Feng Wu, Yan-Ping Li
1Guangdong Medical College, Guangdong Key Laboratory for Research and Development of Nature Drugs, Zhanjiang 524023, China. plliu78@sina.com
Objective:
To investigate the possible mechanisms of nobiletin for anticancer by studying the inhibition effects of nobiletin on tubulin polymerization.
Method:
In vitro nobiletin was added into the tubulin polymerization-depolymerization system and the absorption values were recorded at 350 nm under 37 degrees C.
Result:
As compared with controls, the absorption values in reaction system decreased significantly in nobiletin treatment groups. When nobiletin final concentrations in reaction system were 5.0, 7.5, 10.0 and 12.5 micromol x L(-1), the maximum absorption values were 0 130, 0.109, 0.086 and 0.071 with 16.7%, 30.1%, 44.9% and 54.5% of inhibition rate, respectively. The results suggested that nobiletin could inhibit tubulin polymerization.
Conclusion:
The inhibition effect of nobiletin on tubulin polymerization is the possible mechanism for anticancer.
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