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Updated: Jun 26, 2026

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Published on: December 30, 2021
[Isolation and identification of a thermophilic bacterium from hot springs in Changbai Mountains]
Donglai Liu1, Jingang Cai, Tianyu Yao
1Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, China. ldl198653@126.com
Objective:
We studied physiological and biochemical properties of a thermophilic bacterium isolated from hot springs in Changbai Mountain.
Methods:
The strain CBS-5 (=JCM 15484) from hot springs in Changbai Mountains was isolated by plating and screening on Olive-rich medium. It was characterized by the physiological and biochemical analysis, 16S rDNA sequencing and determination of (G+C)mol% contents.
Results:
The cells were Gram-positive, non-motile rods, spore-forming and generally occurred singly or in pairs. The growth temperature ranges from 48 degrees C to 70 degrees C, optimum at 65 degrees C; growth pH ranges from 6.5 to 8.5, optimum at 7.7. The strain used glucose, sucrose, maltose, lactose and rhanmose as carbon and energy sources. The G+C content of DNA was 41.9 mol%. The major cellular fatty acids were iso-C15:0, C16:0 and iso-C17:0, respectively representing about 24.20%, 20.45% and 17.42% of total fatty acids. The 16S rDNA sequence analysis showed that the strain belonged to the Anoxybacillus genus, for the sequence similarity was 95.1%-98.5% to other known species of Anoxybacillus.
Conclusion:
Strain CBS-5 was a member of Anoxybacillus.
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