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Updated: Jun 26, 2026
13:20
Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel
Kozue Oana1, Yoshiyuki Kawakami, Tetsuya Hayashi
1Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto, Japan.
Microbiology and Immunology
|January 24, 2009
Abstract:
We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.
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