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Updated: Jun 26, 2026

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
Published on: December 13, 2014
Analysis of mRNA decay in Bacillus subtilis
David H Bechhofer1, Irina A Oussenko, Gintaras Deikus
1Mount Sinai School of Medicine of New York University, Department of Pharmacology and Systems Therapeutics, New York, USA.
Abstract:
Studies of mRNA turnover in B. subtilis are less well known than in E. coli. Here we provide researchers who have an interest in gram-positive RNA processing with several protocols for RNA isolation, for 5'- and 3'-mapping of mRNAs and mRNA decay fragments, and we also include a comprehensive listing of B. subtilis mutants that are deficient in ribonucleases thought to be involved in mRNA decay.
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