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Nuclear Export of mRNA

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

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Related Experiment Video

Updated: Jun 26, 2026

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

Measuring mRNA decay in human mitochondria.

Asuteka Nagao¹, Narumi Hino-Shigi, Tsutomu Suzuki

¹Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan.

Methods in Enzymology

|January 24, 2009

Summary

Researchers developed a new method to measure the stability of human mitochondrial messenger RNAs (mRNAs). This technique helps understand how individual mitochondrial mRNAs achieve unique stability levels, crucial for cellular respiration.

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Related Experiment Videos

Last Updated: Jun 26, 2026

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Human mitochondria encode 13 essential proteins for respiratory chain complexes.
Mitochondrial DNA mutations can lead to serious diseases.
Differential mRNA stability, controlled by polyadenylation/deadenylation, influences mitochondrial gene expression.

Purpose of the Study:

To establish a practical method for quantifying human mitochondrial mRNA half-lives.
To investigate the post-transcriptional regulation of mitochondrial gene expression.

Main Methods:

Quantitative real-time reverse transcription PCR (qRT-PCR) was employed.
The method focuses on measuring the decay rates of individual mitochondrial mRNAs.

Main Results:

A practical method for determining human mitochondrial mRNA half-lives was successfully developed.
This method allows for the precise measurement of stability for each mitochondrial mRNA.

Conclusions:

The developed qRT-PCR method provides a valuable tool for studying mitochondrial mRNA stability.
Understanding mRNA half-lives is key to elucidating the molecular mechanisms behind mitochondrial gene expression regulation.