An optimised protocol to prepare Phaeocystis globosa morphotypes for scanning electron microscopy observation
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Published on: February 3, 2017
Natacha Guiselin1, Lucie Courcot, Luis Felipe Artigas
1LOG - UMR CNRS 8187 - Université du Littoral Côte d'Opale, Maison de la Recherche en Environnement Naturel, Wimereux, France. natacha.guiselin@univ-littoral.fr
Journal of Microbiological Methods
|January 24, 2009
Abstract:
A preparation protocol for scanning electron microscopy (SEM) was adapted and tested to the observation of Phaeocystis globosa morphotypes. For extra colonial cells, critical point drying (CPD) gave satisfactory results while for intra colonial cells, preservation with Lugol's iodine and/or glutaraldehyde followed by air-drying appeared as the most suitable method.

