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The salmon gene encoding apolipoprotein A-I: cDNA sequence, tissue expression and evolution.

R Powell1, D G Higgins, J Wolff

  • 1Department of Microbiology, University College, Galway, Ireland.

Gene
|August 15, 1991
PubMed
Summary
This summary is machine-generated.

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Researchers isolated and sequenced Atlantic salmon apolipoprotein A-I (sApoA-I) cDNA. This fish protein shares structural features with human apolipoproteins, indicating conserved functions.

Area of Science:

  • Molecular Biology
  • Comparative Genomics
  • Biochemistry

Background:

  • Apolipoproteins (Apo) are crucial for lipid transport and metabolism.
  • Understanding Apo evolution across species provides insights into gene function and regulation.

Purpose of the Study:

  • To isolate and characterize the apolipoprotein A-I (ApoA-I) cDNA from Atlantic salmon (Salmo salar).
  • To investigate the evolutionary relationships of salmon ApoA-I (sApoA-I) with other known apolipoproteins.

Main Methods:

  • cDNA isolation and sequencing of Atlantic salmon apolipoprotein.
  • Northern-blot analysis to determine gene expression patterns in salmon tissues.
  • Phylogenetic analysis to compare sApoA-I with mammalian apolipoproteins.

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Main Results:

  • A cDNA encoding a 258 amino acid peptide, identified as sApoA-I, was isolated.
  • sApoA-I shares structural features and conserved sequences with human and avian Apo's, with 23% amino acid identity to human ApoA-I.
  • Gene expression was detected in salmon liver, intestine, and muscle.
  • Phylogenetic analysis indicates that mammalian ApoA-I, ApoA-IV, and Apo-E evolved after the divergence of tetrapod and teleost ancestors.

Conclusions:

  • The Atlantic salmon ApoA-I gene has been identified and characterized.
  • Evolutionary analysis suggests that gene duplication events for mammalian ApoA-I, ApoA-IV, and Apo-E occurred after the split between tetrapod and teleost lineages.