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Updated: Jun 26, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
Published on: January 11, 2011
Fiber-optic endomicroscopy system for high-resolution nonlinear imaging of biological tissue
Yicong Wu1, Jiefeng Xi, Michael J Cobb
1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Abstract:
A flexible fiber-optic endomicroscopy system was developed for nonlinear optical imaging of biological samples. Double-clad fiber and photonic bandgap fiber were employed in the endomicroscope for femtosecond pulse delivery, dispersion compensation, nonlinear optical signals collection and fast beam scanning. Three-dimensional imaging of biological tissues based on second harmonic generation and two-photon excited fluorescence was performed with the endomicroscope. The depth-resolved imaging results strongly suggest the potential of this flexible fiber-optic microscope system for real-time assessment of both epithelial and stromal structures in luminal organs.
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