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Updated: Jun 26, 2026

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
Published on: January 6, 2010
Microbubble-mediated oxygen delivery to hypoxic tissues as a new therapeutic device
Agnese Bisazza1, Pierangela Giustetto, Alessandro Rolfo
1Department of Drug Science and Technology, University of Torino, Italy. pbisazz@tin.it
Abstract:
Chitosan-coated oxygen microbubbles of average diameter 2.5 mum, narrow size distribution and spherical shape were prepared. A core-shell structure was evidenced by fluorescence microscopy using fluorescent microbubbles. Such microbubbles can be a therapeutic device for vehiculating oxygen to hypoxic tissues, provided they show proper permeability and diffusivity properties and are non-toxic. Our study proves that oxygen is efficiently delivered both in 'in vitro' and 'in vivo' preparations, and can be conveniently metabolized reversing the cellular hypoxic response. Moreover, toxic effects were investigated in human blood and in cultured cells and no evidence for them was found.
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