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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

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Angular domain optical imaging using a micromachined tunnel array and a Keplerian lens system.

F Vasefi¹, B Kaminska, G H Chapman

¹School of Engineering Science, Simon Fraser University, Burnaby, BC, Canada.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

|January 24, 2009

Summary

Angular Domain Imaging (ADI) uses new micro-tunnels and a Keplerian lens system to improve spatial resolution. This advanced technique can resolve 100 micrometer objects in scattering media.

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Last Updated: Jun 26, 2026

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Area of Science:

Biomedical Optics
Photonics
Microfabrication

Background:

Highly scattering media impede light penetration, limiting imaging depth and resolution.
Traditional imaging techniques struggle to isolate quasi-ballistic photons for clear visualization.
Angular Domain Imaging (ADI) offers a method to select specific photon trajectories.

Purpose of the Study:

To investigate improvements in Angular Domain Imaging (ADI) using novel micromachined micro-tunnels.
To enhance spatial resolution and image definition in scattering media.
To evaluate the performance of ADI with reduced channel spacing and a Keplerian lens system.

Main Methods:

Utilized newly micromachined silicon micro-tunnels with reduced spacing.
Incorporated a Keplerian lens system to mitigate diffracted light from internal reflections.
Employed an 808 nm wavelength laser for illumination.
Tested the system with a 0.3% Intralipid solution and 100 micrometer test structures.

Main Results:

Observed significant improvements in spatial resolution, characterized by sharper edges and enhanced definition.
Successfully resolved test structure objects down to 100 micrometers.
Demonstrated effective removal of diffracted light using the Keplerian lens system.
Validated the enhanced ADI technique in a 2 cm long cuvette.

Conclusions:

The modified ADI technique with novel micro-tunnels and Keplerian optics significantly enhances spatial resolution.
This improved ADI system is capable of imaging fine details within scattering biological tissues.
The findings support ADI as a promising technique for high-resolution imaging in turbid media.